Comparison among Four Method for Diagnosing Rubella Virus in Pregnant Women

Abstract

This study was intended to determine rubella virus seroprevalence in pregnant women. The samples included
240 women who attended during the duration from October 2018 to May 2020 Al-Batool Gynecology
Hospital in Baqubah District. Women’s age ranged from 16-45 years; their age mean was 26.38 years. Data
including age, number of previous abortions. Sera were collected from each woman, and the levels of rubella
IgG and IgM were detected by use Electrochemiluminescence (ECLIA), enzyme-linked immunosorbent
assay (ELISA) and On Site Rubella IgG/IgM Rapid (OnSite) tests. Also, RT-PCR test are used to detect
rubella viral RNA. The results revealed that anti-rubella IgG antibodies for the 240 pregnant women were
ranges from 84.58 % to 89.58 % depending on the method tested used. While the anti-rubella IgM antibodies
for the 240 pregnant women were ranges from 5 % to 5.41 % depending on the method tested used. Only 8
from 240 specimens of pregnant women are rubella viral RNA positive with (3.33 %) percentage by RT-PCR
test. In relation to the history of abortion, the seropositivity of rubella antibodies were (61.42 %) of pregnant
women had history of abortion with significant relation P< 0.05, however the abortion frequency among
Pregnant patients have the past of one or more abortions with the nonsignificant P>0.05 relation.