Molecular Detection of Tumor Necrosis Factor-Alpha (TNF- a) Gene in Mycotic aborted Placenta of Ewes Using RT-PCR

Abstract

Background: Abortion is associated with altered inflammatory molecule expression at the maternal-fetal
interface. (TNF-alpha) are inflammatory proteins and display cytokine as a function that enhances the
leukocyte recruitment to the inflammatory site.
Objectives: This work aimed at establishing the TNF-alpha gene expression in mycotic aborted placental
animals as opposed to normal delivery.
Materials and Method: Using the technique Real Time-Polymerase Chain Reaction (RT-PCR), TNF-?
DNA, tested in the mycotic aborted Placenta group (15 cases), normal delivery group (15 cases).
Results: The levels of TNF-? DNA expressed in the Placenta varied significantly between the two groups
(P<0.05), respectively. The expression of TNF during abortion was significantly higher than in normally
delivered models.
Conclusion: These findings indicate that both cytokines Th1 and Th2 play a key role in the pathogenesis of
abortion. TNF alpha is likely a source of abortion’s genetic susceptibility. There had been a strong correlation
between the two cytokines and abortion.