Molecular Localization of Human Papilloma Viral 16/18 DNA  in Adenoctomized Tissues from a group of Iraqi Patients

Authors

Taghreed F. Almahbobi1 ; Mustafa Jaffar Hussien 2 , Zainab A. Hamid1, Shakir H. Mohammed Al-Alwany3 Saad Hasan Mohammed Ali4

Adenoid hypertrophy; Nasopharyngeal adenotonsillar tissues; HPV 16/18; CISH.

Background: Low- and high- oncogenic risk human papilloma viral infections have been related to the

genesis of a variety of human benign and malignant tumors in the oral cavity and nasopharyngeal tissues.

High- risk types of human papilloma virus are now well established as major etiologic factors of head

and neck cancers, including tonsillar carcinomas. Objective: The current prospective case- control study

aimed to unravel the frequency of HPV genotype 16/18 DNA detection rates in the adenoctomized tissues

from patients with nasopharyngeal tonsillar adenoid hypertrophy. Materials and Method: A sixty (60)

nasal as well as nasopharyngeal adenotonsillar tissues obtained from patients with adenoid hypertrophy via

adenoctomies were enrolled. Forty (40) adenotonsillar tissues were from patients with adenoid hypertrophy,

and (20) normal nasal tissue specimens were obtained from pediatric patients following trimming operations

of inferior nasal turbinates’ with unremarkable pathological changes (as an apparently healthy control

group). A recent version of chromogenic in situ hybridization (CISH) method for HPV detection were

performed by using specified DNA probes for DNA of high- risk HPV 16/18 genotypes. Results: Among

nasopharyngeal adenotonsillar tissues group, 15 out of 40 have revealed positive CISH signals for DNA of

HPV 16 / 18 genotype, constituting 35% of the total screened adenoid hypertrophied tissues. No positive–

CISH reactions were detected in the control nasal tissues. Statistically, the results obtained in this study

showed significant difference when compared to the control tissues group. Conclusions: The significant rate

of such high- oncogenic HPV genotypes detection in those nasopharyngeal adenotonsillar tissues criticizes

searching its further importance, as HPV 16/18 genotypes are critically correlated to many pre- neoplastic

as well as malignant lesions.

Taghreed F. Almahbobi1 ; Mustafa Jaffar Hussien 2 , Zainab A. Hamid1, Shakir H. Mohammed Al-Alwany3 Saad Hasan Mohammed Ali4

1Lecture, Jaber Ibn Hayyan Medical University/ Iraq, 2 Lecture, University of Islamic/ Iraq, 3 Assist. Prof., College

of Science / University of Babylon/ Iraq, 4 Prof., College of Medicine / University of Baghdad/ Iraq