Phytochemical Screening by HPLC and FTIR Spectroscopy of Glucokinin Isolated from Methanol Extract of Bauhinia Variegata

Abstract

The isolation of active plants compounds lead to discover a several drugs derived directly from plants. In the
current study, the Glucokinin was isolated and purified from the methanol leave extract of medicinal plant
Bauhinia variegate, the attempts to identified and characterized it’s functional groups by the HPLC and FTIR
spectroscopy was worked in this study. By using FT-IR spectroscopic the Glucokinin shown different
functional groups. The FTIR spectrum of the purified material, Glucokinin from the methanol leaf extract
of B. variegata indicate the presence of different functional groups. The peak near 1650 cm-1 is the amide
I band.